Anisotropy in Shape and Ligand-Conjugation of Hybrid Nanoparticulates Manipulates the Mode of Bio–Nano Interaction and Its Outcome

Xiaoyou Wang, Li Lin, Renfa Liu, Min Chen, Binlong Chen, Bo He, Bing He, Xiaolong Liang, Wenbing Dai, Hua Zhang, Xueqing Wang, Yiguang Wang, Zhifei Dai and Qiang Zhang. Adv. Funct. Mater. 2017 Aug 18, 27, (31)

    In an attempt to manipulate the biological features of nanomaterials via both anisotropic shape and ligand modification, four types of nanoparticulates with good morphological stability are designed and engineered, including hybrid nanospheres, nanodiscs, and nanodiscs with edge- or plane-modification of octa-arginine (R8) sequence. It is found that the R8 modification anisotropy could trigger huge differences in the endocytosis, intracellular trafficking and even tissue penetration of nanoparticulates. From plane- to edge-modification of R8, the maximum increase in cell uptake reaches to 17 fold, which is much more significant than shape anisotropy alone. On the other hand, 6 types of different cell lines are investigated here to simulate biological microenvironment. It is demonstrated that the maximum difference in cell uptake among 6 cell lines is 12 fold. Three main driving forces are found to contribute to such bio-nano interactions. Based on our findings, it seems possible to manipulate the biointeraction mode of nanomaterials and its output, through regulating their anisotropy both in shape and ligand modification. 

Keywords：delivery manipulation,nanodiscs,ligand-modification anisotropy,shape anisotropy, endocytosis

Pubmed：https://doi.org/10.1002/adfm.201700406